Emergence of Plasmodium falciparum strains with artemisinin partial resistance in East Africa and the Horn of Africa: is there a need to panic?

The emergence and spread of artemisinin partial resistance in East and Horn of Africa is alarming. However, artemisinin-based combination therapy (ACT) generally remains efficacious for the treatment of falciparum malaria. The emergence of partial artemisinin resistance does not currently meet the criteria to initiate change on treatment guidelines nor affect ACT routine procurement and distribution. It is high time for scientists and transitional researchers to be more critical and vigilant on further changes so that national programmes will be able to make informed decisions as well as remain alert and prepared for any change that may be required in the future.

Artemisinin resistance is mediated by mutations in the K13 protein.ArtR is identified by several field validated drug pressure invitro assays as well as genetic engineering studies [13][14][15].These mutations likely alter ubiquitination patterns and help parasites avoid accumulation of polyubiquitinated proteins.Mutations in the propeller domain of the k13 gene are now being routinely validated as markers of artemisinin resistance through the use of CRISPR gene editing and in vitro ring stage survival assay (RSA) [14].However, the independent emergence of multiple partial resistance markers mostly unsupported by TES results makes policy recommendations challenging.
This commentary summarizes the recent data from East Africa and the Horn of Africa (EHoA), with an emphasis in Eritrea and Ethiopia where k13 R622I mutation and pfhrp2/3 deletions co-occur.The main objective of this commentary is to help inform the national malaria control programme (NMCP) and policymaker decision-making.

Southeast Asia (SEA)
Clinical artemisinin resistance was first demonstrated in two parallel studies conducted in Western Cambodia in the late 2000s [2,3].Patients were identified with continued infection for up to 7 days after artesunate monotherapy and decreased parasite clearance rates.While propeller domain initially remained effective in SEA, ArtR emergence set the stage for ACT failure owing to rapid emergence of partner drug resistance [6].In many areas, 35-45% of patients treated with ACT failed therapy due to the combined effect of artemisinin and partner drug resistance [12].
However, ACT remain efficacious in Africa (for now).Therapeutic efficacy studies show ACT efficacy > 95% in most settings, well above the WHO 90% threshold for policy change.However, there is strong evidence that WHO-validated and candidate k13 mutations associated with ArtR are emerging in Africa and beginning to spread.This includes R561H in Rwanda, Uganda and Tanzania; C469Y and A675V in Uganda [5,6,21]; and R622I in Ethiopia and Eritrea (Table 2).Recent study showed high prevalence of R622I in northern Ethiopia [8].This mutation was first reported in two small studies in the northwest part of Ethiopia (North Gondar) with increasing prevalence over 5 years, 2.4% in 2013 and 9.5% in 2017-18 [22].
In Ethiopia, AL has been used for uncomplicated P. falciparum malaria since 2005.Accordingly, several TES have been conducted per WHO requirement, mainly led by the Ethiopian Public Health Institute (EPHI) and other groups.The results have been encouraging, with an average adequate clinical and parasitological response  (ACPR) of approximately 96% The studies were conducted for programmatic purposes, and the data has been utilized by the NMCP to guide decision making and disseminated in peer-reviewed journals.However, major publications by Fola et al. [8], and Mihreteab et al. [9] reported established and expanding high prevalence of ArtR mutations (R622I) in Ethiopia and Eritrea, findings supported by a recent manuscript by Emiru et al. [17] from Ethiopia.These findings are alarming and surprising local programmes as they contradict high ACT efficacy observed during TES.

Implications
Reports of mutations associated with ArtR are relevant to the programme and require a careful response.Multiple WHO-candidate and -validated ArtR markers have been reported across EHoA (Table 3), with k13 622I prevalent in Ethiopia and Eritrea.Validated makers are known to improve parasite survival in vitro during RSA, as well as delayed parasite clearance in vitro or in clinical cases assessed by day 3 parasitaemia [23].The k13 R622I mutation currently reported in Ethiopia and Eritrea is a WHO-validated marker.Because R622I prevalence varies from place to place within these countries and sampling was not nationally representative, nationwide genomic surveillance is warranted.Sampling across different eco-epidemiological transmission zones is important to understand the distribution of ArtR markers.The Ethiopian study by Fola et al. [8] used clinic-based sampling and built upon EPHI's work on pfhrp2/3 deletion [24].However, the Eritrean study byMihreteab et al. came from three consecutive TES surveys in 2016, 2017, 2019 [9].The results indicate that ACT efficacy is threatened.However, the WHO definition of artemisinin partial resistance requires delay in the clearance of malaria parasites from the bloodstream or in an in vitro setting after ACT challenge.Patients infected by P. falciparum with partial resistance ACT may harbour parasites for more than 3 days after treatment.The remaining parasites have a better chance of survival and onward transmission unless partner drugs that remain in circulation are capable of killing them [2,3].Killing by partner drugs with longer half-life than artemisinin derivatives may explain the high efficacy of ACT despite partial resistance; these patients treated with an artemisinin-based combinations are fully cured if the partner drug is still efficacious [23].
In summary, the emergence of artemisinin partial resistance in EHoA requires immediate and more expansive clinical and genomic surveillance to closely monitor the extent of resistance and determine its clinical impact.
Despite the multiple emergences of partial resistance molecular markers, ACT remain efficacious in Ethiopia and fulfill WHO requirements for the treatment of uncomplicated P. falciparum malaria.Therefore, the emergence of partial artemisinin resistance does not currently meet criteria for changes in routine treatment of patients with ACT or affect routine procurement and distribution of ACT.Researchers and scientists need to work together to generate deeper and wider studies that are representative and monitor closely for evidence of decreased clinical efficacy of ACT.While increasing reports of ArtR mutations are alarming, careful and conscientious decisions will allow the programme to remain alert and to prepare for future policy changes if needed.

Take home message to national malaria control programmes: do not panic but remain vigilant!
-Recent reports of ArtR should alert the programme to the need for robust, representative phenotypic and genomic surveillance.Genomic surveillance may need to be integrated into ongoing routine TES activities.-Alternative artemisinin-based combinations and second-line drugs need to be sought and procurement plans developed.-Lessons from Southeast Asia need to be reviewed and used to control the spread of ArtR parasites and for mitigation.-Currently available data is in its early stage.Recent reports should generate concern but not panic.The programme does not need revise its current treatment regimen, nor should health providers hesitate to continue routine use of ACT.However, the programme has an opportunity to be proactive against the threat of ArtR, including close monitoring and

Table 1
High ACT therapeutic efficacy for uncomplicated P. falciparum malaria across sentinel sites in Ethiopia Studies conducted by the Ethiopian Public Health Institute (EPHI) for the NMCP since 2005 are included

Table 2
K13 mutations recently reported in East Africa

Table 3
WHO K13 validated and candidate markers for ArtR alternative therapies should they be required in future.-Theprogramme and researchers must work together to enable evidence-based decision-making.-Mapping partner drug resistance needs to be expedited, as their efficacy is a key reason why ACT remains effective in regions affected by ArtR P. falciparum.